A tertiary-branched tetra-amine, N 4 -aminopropylspermidine is a major cellular polyamine in an anaerobic thermophile, Caldisericum exile belonging to a new bacterial phylum, Caldiserica Abstract Acid-extractable cellular polyamines of thermophilic Caldisericum exile belonging to a new bacterial phylum, Caldiserica were analyzed by HPLC and GC. The coexistence of an unusual tertiary brancehed tetra-amine, N 4 -aminopropylspermidine with spermine, a linear tetra-amine, as the major polyamines in

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... addition to putrescine and spermidine, is first reported in the moderate thermophile isolated from a terrestrial hot spring in Japan. Linear and branched penta-amines were not detected. The novel cellular polyamine profile found in the moderate thermophile has never been observed within various bacteria and archaea previously analyzed.