A Systematic Screen for Protein-Lipid Interactions in Saccharomyces cerevisiae [unknown]

O. Gallego, C. Fernandez-Tornero, C. Aguilar-Gurrieri, C. Muller, A.C. Gavin
2010 unpublished
Protein-metabolite networks are central to biological systems, but are incompletely understood. Here, we report a screen to catalog protein-lipid interactions in yeast. We used arrays of 56 metabolites to measure lipid-binding fingerprints of 172 proteins, including 91 with predicted lipid-binding domains. We identified 530 protein-lipid associations, the majority of which are novel. To show the data set's biological value, we studied further several novel interactions with sphingolipids, a
more » ... s of conserved bioactive lipids with an elusive mode of action. Integration of live-cell imaging suggests new cellular targets for these molecules, including several with pleckstrin homology (PH) domains. Validated interactions with Slm1, a regulator of actin polarization, show that PH domains can have unexpected lipid-binding specificities and can act as coincidence sensors for both phosphatidylinositol phosphates and phosphorylated sphingolipids. Molecular Systems Biology 6: 430;
doi:10.2210/pdb3nsu/pdb fatcat:y55zuvjbtjfajargjuo3jcjlfe