Preliminary Study on Acyl Incorporation and Migration in the Production of 1,3-diacylglycerol by Immobilized Lipozyme RM IM-catalyzed Esterification

Zong Meng, Shan Lu, Wenxin Geng, Jianghua Huang, Xingguo Wang, Yuanfa Liu
2014 Food science and technology research  
The acyl migration in the production of 1,3-diacylglycerol (1,3-DAG) by immobilized lipozyme RM IM-catalyzed esterification reaction in a solvent-free system was investigated, particularly including the acyl migration of 1,3-DAG to 1,2-diacylglycerol (1,2-DAG), which affected by some of the system's parameters (substrate molar ratio, enzyme load and reaction temperature). The results showed that all parameters had strong positive influences on acyl migration. With the increasing substrate molar
more » ... ratio of oleic acid to glycerol from 1:1 to 3:1, the acyl migration showed a decreasing-increasing pattern. However, the degree of acyl migration increased gradually concomitant with the increasing of enzyme load (2 _ 10 wt% of substrates) and reaction temperature ( 45 _ 75℃). The conditions for the highest yields of 1,3-DAG and the relatively low levels of acyl migration were optimized as follows: temperature 65℃, oleic acid/glycerol molar ratio 2:1, enzyme load 6%, reaction time 2 h, 0.01 MPa vacuum, 200 rpm stirring speed.
doi:10.3136/fstr.20.175 fatcat:oc3w6cp2wbawvgmjdbrk2lz2i4