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Folia Endocrinologica Japonica
We have developed a preparation of monolayer cultures of bovine parathyroid cells in order to elucidate the control mechanism of the biosynthesis and secretion of parathyroid hormone (PTH) at cellular level. Dispersion of parathyroid cells was performed by stirring minced bovine parathyroid tissues in Hanks' BSS containing 0.3-0.5% collagenase at 37°C for 60 min. Dispersed cells were cultured at 37°C in MEM•Hanks' BSS containing 10% fetal calf serum and 15 mM HEPES. On the 5th day of thedoi:10.1507/endocrine1927.52.11_1063 pmid:201510 fatcat:qdsrcyhvmjhe3j35lrvzsf5kue