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The aim of this study is to investigate the mechanistic pathway of the oxidation of L-ascorbic acid by periodate ions in acidic medium reaction. The oxidation of L-ascorbic acid (H 2 A) by periodate ions in acidic medium has been investigated under pseudo-first order conditions at 27 ± 0 o C, [H + ] = 1.0 x 10 -3 mol dm -3 , I= 1.0 mol dm -3 (NaCl) and λ max 430nm. The stoichiometry of the reaction was observed to be 2:7 in terms of mole ratio of periodate ions and ascorbic acid consumed. Thedoi:10.5296/jbls.v4i2.3349 fatcat:h6iotja435dlbfnn6aoy2dlfse