Characterisation of biotinylated liposomes for in vivo targeting applications

Helen C. Loughrey, Anita Ferraretto, Ann-Marie Cannon, Giulia Acerbis, Francesco Sudati, Giovanni Bottiroli, Massimo Masserini, Marco R. Soria
1993 FEBS Letters  
Liposomes containing monosialoganglioside (G") or polyethylene glycol (PEG) lipid derivatives have prolonged circulation in the blood. This favours liposome extravasation to tumour sites. In this report it is shown that inclusion of G", PEGS,-DPPB or PEGmxrDPPE in liposomes containing biotin-DPPE signiticantly diminished the ability of vesicles to bind to streptavidin in vitro. Steric inhibition due to the bulky head group of these lipids was least for biotin-DPPE liposomes contaming C&i.
more » ... bu~on studies in CZ6 t~o~-~a~ng mice showed that ~~~-~~sornes containing small amo~ts of biotin-DPPE have long circulation l&-times in the blood. Using fluorescent microscopic techniques, liposomes contaming both G" and biot~-DPPE were detected within ex~-vault spaces in tumours. In addition it was shown that biotin-DPPE in G&iposomes bound streptavidin in situ. These results suggest that G"-liposomes containing biotin-DPPE have potential use as diagnostic or therapeutic reagents in pre-targeting applications dependent on the high-a&&y interaction of biotiu with streptavidin.
doi:10.1016/0014-5793(93)80509-s pmid:8405439 fatcat:rdzv5unv6ffmfevmyfw7rqtpty