A B S T R A C T An isocratic reverse phase High Performance Liquid Chromatographic (RP-HPLC) stability indicating method was developed and validated for determination of Assay of Caspofungin in Caspofungin acetate for injection. The successful determination of Caspofungin was achieved using YMC-Pack Polyamine II (150×4.6 mm i.d., 5µ particle size) column maintained at 30°C temperature with mobile phase consisting 0.02 M phosphoric acid buffer of pH 3.5, acetonitrile and 2-propanol in an

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... c method. The mobile phase flow rate was 1.0 mL/min and the detection wavelength was 210 nm. The developed RP-HPLC method was validated according to ICH guidelines with respect to linearity, accuracy, precision, specificity and robustness. Validation studies demonstrated that the proposed method is simple, specific, rapid, reliable and reproducible, and this method can be applied for the routine quality control analysis of Caspofungin in Parenteral dosage form.