Multiplatform serum metabolic phenotyping combined with pathway mapping to identify biochemical differences in smokers

Manuja R Kaluarachchi, Claire L Boulangé, Isabel Garcia-Perez, John C Lindon, Emmanuel F Minet
2016 Bioanalysis  
Aim: Determining perturbed biochemical functions associated with tobacco smoking should be helpful for establishing causal relationships between exposure and adverse events. Results: A multiplatform comparison of serum of smokers (n = 55) and never-smokers (n = 57) using nuclear magnetic resonance spectroscopy, UPLC-MS and statistical modeling revealed clustering of the classes, distinguished by metabolic biomarkers. The identified metabolites were subjected to metabolic pathway enrichment,
more » ... ling adverse biological events using available databases. Perturbation of metabolites involved in chronic obstructive pulmonary disease, cardiovascular diseases and cancer were identified and discussed. Conclusion: Combining multiplatform metabolic phenotyping with knowledge-based mapping gives mechanistic insights into disease development, which can be applied to next-generation tobacco and nicotine products for comparative risk assessment.
doi:10.4155/bio-2016-0108 pmid:27635669 fatcat:fbjfuavcdzeslbxlas24j3nujm