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Human Fibroblast Intracellular Network Prepared with Digitonin for Field Emission Scanning Electron Microscopy
1992
Archives of histology and cytology
Results obtained by extracting human fibroblast cells for the study of cytoskeletal structures are compared. Cells grown in culture were treated with digitonin in contrast to other methods of detergent preparation using Triton and Saponin. The three dimensional intracellular network which resulted from digitonin treatment was found to be similar in appearance to the structures observed by high voltage transmission electron microscopy of untreated cells described as a microtrabecular lattice by
doi:10.1679/aohc.55.suppl_57
pmid:1290677
fatcat:cjtile5tx5d7vgqgfawqwofm3y