Impact of Hymenoptera venom allergy and the effects of specific venom immunotherapy on mast cell metabolites in sensitized children
Annals of Agricultural and Environmental Medicine
Cichocka-Jarosz E, Sanak M, Szczeklik A, Brzyski P, Pietrzyk JJ. Impact of Hymenoptera venom allergy and the effects of specific venom immunotherapy on mast cell metabolites in sensitized children. Abstract Introduction and objective. Mast cells (MC) are effector cells during severe systemic reactions (SR) to Hymenoptera stings. Venom specific immunotherapy (VIT) is the treatment of choice for prevention of SR to stings. Tryptase and prostaglandin D 2 metabolites (PGD 2 ) are the markers of MC
... ctivation. The study design was to 1. compare baseline values of serum tryptase concentration (BST) and PGD 2 metabolites in children with/without venom sensitization, 2. to evaluate an influence of rush VIT on MC markers in treated children. Materials and methods. Sensitized group: 25 children with SR to Hymenoptera sting. Control group: 19 healthy children. Active treatment: 5-day-rush-VIT. BST was evaluated by ImmunoCAP, PGD 2 metabolites in blood and urine by GC-NICI-MS. Results. The baseline blood levels of MC markers were significantly higher, while urinary concentration of 9α,11β-PGF 2 was significantly lower in the whole group of venom-sensitized children compared to controls. Severity of SR showed negative correlation with urinary PGD 2 metabolites, while positive with plasma 9α,11β-PGF 2 and BST concentration The highest sensitivity was obtained for plasma 9α,11β-PGF 2 whereas the highest specificity for urinary PGD-M. Conclusions. In children with IgE-mediated SR to Hymenoptera stings, elevation of baseline values of PGD 2 metabolites in blood is accompanied by decreased excretion of its urinary metabolites. Assessment of stable PGD 2 metabolites might serve as an independent MC marker to identify allergic children. There is an association between urinary PGD 2 metabolites and severity of the SR to Hymenoptera stings.