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Trial LC-MS analysis with a short gradient time for proteomics ofmultiple samples
日本プロテオーム学会/短時間の質量分析計解析で多数のタンパク質を同時に測定する試み
2019
日本プロテオーム学会/短時間の質量分析計解析で多数のタンパク質を同時に測定する試み
Preserved Fulltext
Proteomes of tissues or body fluids have been analyzed by LC-MS. For comprehensive analysis of the proteome, well-separation of peptides in LC with a long gradient time, such as 2 to 3 hours has been utilized to identify a larger number of proteins in a sample. However, as the long gradient time makes the throughput low, we tried to analyze by LC-MS in a short gradient time such as 30 to 45 min to know how the comprehensiveness may disturbed by using Bruker TomsTOFpro for MS and nanoElute or
doi:10.14889/jhupo.2019.0.227.0
fatcat:3bh3vtqcc5cbpajq6kmasrgyz4