Glaucoma is associated with diabetes. Metformin is one of the first therapeutic options for diabetes and may act to overcome the incidence or severity of glaucoma. Study objectives: Tto study the prevalence of glaucoma in Jordanian diabetic patients and to investigate the association of glaucoma and metformin treatment in diabetic Jordanian patients. Methods and subjects: Aretrospective study was conducted to collect data from files of diabetic patients who attended internal medicine clinics at

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... Royal Medical City. Files were eligible if the status of glaucoma was available and if the treatment with metformin was indicated.Dataincluded demographic variables such as age and gender; and medical conditions such as diabetes, glaucoma, and metformin treatment. Data was analyzed using SPSS version 20. Data was presented as frequencies, percentages, means and standard deviation. The relationship between variables was investigated using Chi-Square, and One way Anova. Significance was considered at alpha <0.05. Study findings showed that the prevalence of glaucoma was 50% among diabetic patients. Study findings showed that the prevalence of glaucoma was 50% among diabetic persons. Glaucoma was not associated significantly with metformin use, gender, age, metformin use, or metformin dose. Taken together, the present study showed that the glaucoma is not associated significantly with study variables included in the study.