Single-Nucleotide Resolution of N6-Adenine Methylation Sites in DNA and RNA by Nitrite Sequencing

Yasaman Mahdavi-Amiri, Kimberley Chung Kim Chung, Ryan Hili
2020 Chemical Science  
A single-nucleotide resolution sequencing method of N6-Adenine methylation sites in DNA and RNA is described. Using sodium nitrite under acidic conditions, chemoselective deamination of unmethylated adenines readily occurs, without competing...
doi:10.1039/d0sc03509b pmid:34163791 pmcid:PMC8179008 fatcat:lspishykbvhhnciu6h6x5dnqv4