An actinomycete strain, designated IR27-S3 T , was isolated from a forest soil sample collected from Iriomote Island, Okinawa, Japan. Cells of the isolate were Gram-stain-positive, aerobic, nonsporulating, non-motile coccoids or short rods. The strain grew in the presence of 0-7 % (w/v) NaCl, at pH 6-8 and at 12-37 6C, with optimum growth at 30 6C. Chemotaxonomically, the strain contained LL-diaminopimelic acid as the diagnostic diamino acid and MK-8(H 4 ) as the predominant menaquinone. The

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... ar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The major cellular fatty acids were iso-C 16 : 0 , C 17 : 1 cis-9, C 15 : 0 and iso-C 15 : 0 . The DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence analysis, strain IR27-S3 T was closely related to Nocardioides mesophilus MSL-22 T (98.1 % 16S rRNA gene sequence similarity), Marmoricola bigeumensis MSL-05 T (97.2 %) and Nocardioides jensenii DSM 20641 T (96.5 %). On the basis of fatty acid analysis, phylogenetic analysis and phenotypic data, the isolate should be classified in a novel species of the genus Nocardioides, for which the name Nocardioides iriomotensis sp. nov. is proposed. The type strain is