Hard tissue formation induced by synthetic oligopeptide derived from an enamel matrix derivative

Michiko Noguchi, Kazuya Tominaga, Akio Tanaka, Masatoshi Ueda
2012 Oral Medicine & Pathology  
Eosinophilic round bodies (ERBs) are composed of fragments of amino acid sequences of varying lengths, which represent a portion of exon 5 of amelogenin gene. ERBs have previously been shown to accumulate in the backs of rats subcutaneously injected with the product Emdogain®. In the present study, an oligopeptide with an amino acid sequence similar to this portion was artificially synthesized, and its effects were examined. Methods: The peptide solution was applied to periodontal defects
more » ... d on the maxillae of rats. Sections from these tissues were histopathologically and immunohistochemically examined at 7 and 14 days after the start of the experiment. Results: In the experimental group, hard tissue had formed on the surface of exposed dentin, and both osteopontin and collagen type I (COLI) were localized around the hard tissue. The hard tissue was stained with periodic acid-Schiff (PAS) more deeply than was the dentin, and the fibers that were positive for COLI were vertically inserted in the rim of the newly-formed hard tissue. No hard tissue formation was found in the control group. Conclusion: These results suggest that synthetic oligopeptide administration can induce the formation of hard tissue, such as cementum, on the periodontal defects of rats.
doi:10.3353/omp.16.75 fatcat:e5osfe4xn5evffg3um7dumgbqi