The total 18 fungal sp. were isolated from rhizoplane and rhizosphere soil of Madhuca indica. Fungi present in the rhizoplane and rhizosphere soil of Madhuca indica were isolated by serial dilution method and identified on the basis of morphological characters. The fungi were Aspergillus niger, A. fumigatus, A. clavatus, Mucor hiemalis, Alternaria triticina, rstolonifer and Mucor racemosus were present in both the rhizoplane and rhizosphere soil where P. oxalicum, A. herbarum and Penicillium

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... quentans A. clavatus, Penicillium chrysogenum species only present in rhizosphere soil but they were absent in rhizoplane soil. The rhizosphere soil contains more fungal species than the rhizoplane. The dominent fungal species were used for the antibacterial activity. Aspergillus niger and Mucor hiemalis fungal culturature were extracts and used for the antibacterial activity against the Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Xanthomonas citri, Ralstonia solanacearum. The maximum zone of inhibition Mucor hiemalis shows 21 mm against Ralastonia solancearum and the Mucor hiemalis minimum zone of inhibition against Bacillus subtilis as compare Mucor hiemalis.