Advances in Triterpenoid Saponins Research 2007-2012
Herbal Medicine Open Access
Triterpenoid saponins isolated and identified from natural resources during the period April, 2007-December, 2012 are reviewed. Current techniques used in their isolation and structure determination are discussed. New triterpenoid saponins isolated along with their occurrence, selected physical data, spectroscopic methods used for their identification and the 13 C NMR data of the novel aglycones of the parent saponins are compiled. The biological properties of those molecules are also included.
... are also included. Green corrosion inhibition effects of saponins and biosynthesis of triterpenoid saponins and production of triterpenoid saponins by tissue culture of those molecules are also discussed. 2 were refluxed four times with 60% EtOH. The dried EtOH extract was applied to a DA101 column and eluted successively with H 2 O, 70% EtOH and 95% EtOH. The saponin fractions thus obtained were repeatedly separated by MPLC with a column of reversedphase silica followed by HPLC . The cytotoxic triterpene glycosides from the Malagasy plant Physena sessiliflora were isolated by Inoue et al. . The dried leaves were boiled with MeOH. After filtration, the extract was evaporated to dryness. The extract was partitioned between equal volumes of n-hexane and MeOH-H 2 O (7:3). Methanol was removed from MeOH-H 2 O layer. The aqueous layer was further partitioned with EtOAc and then n-BuOH successively. The n-BuOH fraction was chromatographed on silica gel and finally purified by HPLC and MPLC on an ODS column to give eight triterpene glycosides. The dried and powdered whole plants of Echinopsis macrogona were extracted with CHCl 3 and MeOH under reflux. The MeOH extract was applied to a Diaion HP-20 column and washed with H 2 O, MeOH-H 2 O (3:7) and 100% MeOH. The MeOH eluted fraction was subjected to column chromatography with various solvent systems to give eight fractions. The fractions were further purified by repeated ODS and silica gel column chromatography using various solvent systems followed by HPLC to afford pachanosides C1, E1, F1 and G1, and bridgesides A1, C1, C2, D1, D2, E1 and E2  . Gao et al. isolated four new triterpenoid saponins from Patrinia scabiosaefolia in another example. The dried plants were extracted with methanol overnight at room temperature. The MeOH extract was partitioned against EtOAc and H 2 O. The aqueous part was applied to HPD 450 macroporous resin column and washed with H 2 O and EtOH. The EtOH eluate was purified by vacuum low-pressure column chromatography on silica gel using CHCl 3 -MeOH in various ratios to afford seven fractions. Rechromatography of the fractions over ODS-C18 followed by repeated MPLC and further HPLC purification furnished four triterpenoid saponins  .