EVALUATION AND OPTIMIZATION OF ANTIOXIDANT POTENTIALITY OF XYLARIA FEEJEENSIS HMJAU22039

Durga Pallavi Rebbapragada, Rajagopal Kalyanaraman
2016 Asian Journal of Pharmaceutical and Clinical Research  
<p>ABSTRACT<br />Objective: Antioxidants neutralize free radicals generated in the human body and prevent them from causing damage. The present study is aimed to<br />evaluate and optimize the antioxidant activity of Xylaria feejeensis HMJAU22039 an endophyte isolated from Tectona grandis.<br />Methods: The antioxidant potential was measured by 1-1diphenyl-2-picryl-hydrazil (DPPH) radical scavenging assay and nitric oxide (NO) scavenging<br />assay. Total phenolic content (TPC) of the samples
more » ... C) of the samples was measured by Folin–Ciocalteu reagent based assay, and the values were obtained from the<br />regression equation: y = 0.006141x + 0.157733.<br />Results: Potato dextrose yeast extract broth medium was selected as the basal medium as it reported a high antioxidant activity. The basal medium<br />was optimized with 2% dextrose, 0.2% yeast extract, and 200 g/L potato infusion. Dextrose and yeast extract were selected as carbon and nitrogen<br />sources, respectively, as they reported high antioxidant activity. The antioxidant activity and phenolic content are highest at static condition<br />(18.14 mg/g). Incubation temperature of 30°C and pH of the initial medium at 6 were found to be optimum conditions for high antioxidant activity. An<br />incubation period of 20 days reported the highest antioxidant activity and phenolic content. Methanol extract recorded high antioxidant activity with<br />a DPPH (73.86%) followed by ethyl acetate and chloroform fractions.<br />Conclusion: The study highlights the importance of different physiochemical parameters in the production of secondary metabolites having<br />antioxidant properties. The results reveal a significant positive correlation between DPPH radical scavenging assay, NO scavenging assay, and TPC.<br />Keywords: 1-1diphenyl-2-picryl-hydrazil, Total phenolic content, Endophytic fungus, Tectona grandis.</p>
doi:10.22159/ajpcr.2016.v9s2.13734 fatcat:2ssd4fqtavbtdpxrj3gwlizy7q