Real-Time Imaging of Bacteria/Osteoblast Dynamic Coculture on Bone Implant Material in an in Vitro Postoperative Contamination Model [component]

Biomedical implants are an important part of evolving modern medicine but have a potential drawback in form of post-operative pathogenic infection. Accordingly, the "race for surface" combat between invasive bacteria and host cells determines the fate of implants. Hence, proper in vitro systems are required to assess effective strategies to avoid infection. In this study we developed a real time observation model, mimicking post-operative contamination, designed to follow E. coli proliferation
more » ... coli proliferation on titanium surface occupied by human osteoblastic progenitor cells (STRO). This model allowed us to monitor E. coli invasion of human cells and titanium surface coated and uncoated with fibronectin. We showed that the surface colonization of bacteria is significantly enhanced on fibronectin coated surfaces irrespective of if areas were uncovered or covered with human cells. We further revealed that bacterial colonization of the titanium surfaces is enhanced in co-culture with STRO cells. Finally, this co-culture system provides a comprehensive system to describe in vitro and in situ bacterial and human cells and their localization but also to target biological mechanisms involved in adhesion as well as in interactions with surfaces, thanks to fluorescent labelling. This system is thus an efficient method for studies related to design and function of new biomaterials. 2 KEYWORDS Real-time imaging, bacteria/eukaryotic cells co-culture, biomaterials, confocal fluorescence microscopy, fibronectin
doi:10.1021/acsbiomaterials.9b00050.s001 fatcat:oyztfavxurdgfbxavfqqvwwdra