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To obtain cytochrome c3 labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with N2 and under pH control. DvMF was able to go on a defined medium without natural products. The composition of medium containing a small amount of NH4Cl as sole nitrogen source was established. Then, uniformly 15 Nlabeled cytochrome c3 was obtained during the culture of DvMF in a defined medium with 15 NH4Cl; it was confirmed by 1 H-15 N HMQC.doi:10.5012/bkcs.2005.26.2.278 fatcat:svhujlwrajeytexu5znh6aglzu