Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. pauca

Paulo Teixeira Lacava, Maria Estela Silva-Stenico, Welington Luiz Araújo, Ana Valéria Colnaghi Simionato, Emanuel Carrilho, Siu Mui Tsai, João Lúcio Azevedo
2008 Pesquisa Agropecuária Brasileira  
The objective of this work was to study the production of siderophores by endophytic bacteria Methylobacterium spp., which occupy the same ecological niche as Xylella fastidiosa subsp. pauca (Xfp) in citrus plants. The siderophore production of Methylobacterium strains was tested according to chromeazurol agar assay test (CAS), Csáky test (hydroxamate-type) and Arnow test (catechol-type). In addition, the ability of Xfp to use siderophores, in vitro, produced by endophytic bacteria as source of
more » ... iron, was evaluated. All 37 strains of Methylobacterium spp. tested were CAS-positive for siderophore production. Methylobacterium spp. produced hydroxamate-type, but not catechol-type siderophores. In vitro growth of Xfp was stimulated by the presence of supernatant siderophores of endophytic Methylobacterium mesophilicum.
doi:10.1590/s0100-204x2008000400011 fatcat:b5uzfukxwnhcfmdf2dfnwjzbnm