| Caseous lymphadenitis (CLA) is a chronic contagious disease of sheep and goats worldwide. It is caused by a bacterium known as Corynebacterium pseudotuberculosis and vaccination is the most suitable method of control. This study measures the humoral and cell-mediated immune responses following vaccination with a recombinant CLA vaccine with palm-based oil as adjuvant. Twenty-five adult rats were divided into 5 groups. Group 1 was vaccinated intramuscularly with recombinant CLA vaccine without

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... adjuvant, groups 2, 3 and 4 were vaccinated with the same vaccine containing 3%, 5% and 7% of palm-based oil, respectively while groups 5 was injected with PBS. The immunoglobulin and cell-mediated immune status were measured for a period of 10 weeks. Rats vaccinated with recombinant vaccine without adjuvant showed slightly high but short response of antibody, CD4+ and CD8+. Rats vaccinated with the recombinant vaccine containing 3% palm oil adjuvant showed significantly (p<0.05) highest and lasting antibody levels and percentages of CD4+ and CD8+ cells. Hence, it is concluded that recombinant CLA vaccine with 3% palm oil adjuvant has potential to be developed as a CLA vaccine. Citation | Roslindawani MN, Syafiqah AS, Jesse FFA, Effendy AW, Zamri-Saad M (2016). Recombinant caseous lymphadenitis vaccine with palm oil as adjuvant enhances the humoral and cell-mediated immune responses in rat model.