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Protocol 1: Electroporation of Agrobacterium tumefaciens with a plasmid of interest v1
[post]
2022
Preserved Fulltext
Electroporation is a widespread method of transforming competent Agrobacterium tumefaciens (Agro) cells with a plasmid containing a T-DNA of interest. The resulting Agro can be used to transform various plants and fungi, resulting in transformed cell lines. This protocol outlines the standard electroporation protocol we use to transform Agro in preparation for Agrobacterium-mediated transformation of the chytrid fungus Spizellomyces punctatus.
doi:10.17504/protocols.io.n2bvj88nwgk5/v1
fatcat:m75p2nheujetvc7yntlu527fci