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Supplemented sawdust with different times of watering has profound effect on chemical composition of Pink Oyster mushroom. Three times watering per days on mushroom had highest amount of carbohydrate (42.42%) and lipid (4.66%) whereas moisture (90.39%), dry matter (10.01%) and crude fiber (21.81%) was highest at watering frequency of four times. Protein content was highest (32.37%) at two times per days of watering. Mineral content of the fruiting body found to be significantly varied onfatcat:zl46pdk5gvaerlzdfz7ksompsq