The Effect Of A Proteinase Inhibitor On Platelet Function In Stored Blood
Thrombosis and Haemostasis
Blood platelets are markedly traumatized by the withdrawal of blood from the donor. Hereby the function of thrombocytes is activated and this platelet stimulation is closely related with the early formation of microaggregates in stored blood. Therefore it is clinically desired to stabilize the platelets but without inhibiting their function irreversibly and causing hemorrhage. Concerning this question an experimental study was carried out.Blood was drawn from 10 volunteers under blood bank
... der blood bank conditions and was stored in presence of 200 KIU aprotinin per ml ACD-blood resp.the same volume of saline at a temperature of +4°C. Immediately after the withdrawal of blood and 1,2,3 and 7 days later blood samples were taken and the following parameters were studied: platelet aggregation induced by ADP, aggregate ratio, PF 4, beta-thromboglobulin and tx B2 in plasma and serum.In the aprotinin blood the aggregability was slightly diminished and was longer present than in the control group. In addition, the aggregate formation was significantly decreased. The release reaction of platelets was not effected by aprotinin;the increase of PF 4 and beta-thromboglobulin was similar to that of the control group. Also the thromboxane formation was not effected. Thus the protective effect of aprotinin is independent from the prostaglandin metabolismThis study shows that a high dose of aprotinin has a stabilizing effect on platelets in stored blood and that the thrombocyte function is not irreversibly inhibited by this substance. Therefore, aprotinin can be regarded to be effective in the prophylaxis of disseminated platelet aggregation without causing a bleeding risk.