ACE2 gene transfer attenuates hypertension-linked pathophysiological changes in the SHR

Carlos Díez-Freire, Jorge Vázquez, María F. Correa de Adjounian, Merari F. R. Ferrari, Lihui Yuan, Xeve Silver, Raquel Torres, Mohan K. Raizada
2006 Physiological Genomics  
MATERIALS AND METHODS Cloning of murine ACE2 in lentiviral vector and production of lenti-mACE2 viral particles. We have cloned the membrane-bound form of murine ACE2 (ACE2). Complementary DNA encoding Mus musculus ACE2 (21, 26) was used as a template for a PCR amplification reaction with the following primers: ACE2 NheI, sense 5Ј-AAGCTAGCATAGCCAGGTCCTCCTGGCTCCTTC-3Ј; ACE2 Article published online before print. See web site for date of publication
doi:10.1152/physiolgenomics.00312.2005 pmid:16788004 fatcat:fzenm3thmrd2zol4h7gvmmkk4y