itively influenced gene expression whereas longer exposures decreased mRNA accumulation. Fluridone, To study the regulation of gene expression during olive an inhibitor of ABA synthesis and accumulation, (Olea europaea) fruit development, a full length cDNA decreased mRNA levels while ABA added to fluridone encoding the stearoyl-ACP desaturase (EC 1.14.99.6), supplemented media restored D9-desaturase tranwhich catalyses the conversion of saturated stearic script accumulation. These results

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... st that acid to mono-unsaturated oleic acid was isolated. The D9-desaturase gene expression in olives is temporally primary structure of the protein as deduced from the and developmentally regulated in olive fruit. nucleotide sequence of the cDNA includes a putative 27-amino acid transit peptide. Amino acid sequence Key words: ABA induction, developmental regulation, comparison showed substantial similarity to D9-desatdesaturase, gene expression, olive. urases isolated from other plant species. The two conserved motifs D/EEXXH, involved in the catalytic reaction are localized in similar positions to the other