Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres [post]

Patricia P Garcez, Juliana Minardi Nascimento, Janaina Mota de Vasconcelos, Rodrigo Madeiro da Costa, Rodrigo Delvecchio, Pablo Trindade, Erick Correia Loiola, Luiza M. Higa, Juliana Cassoli, Gabriela Vitória, Patricia Sequeira, Jaroslaw Sochacki (+7 others)
2016 unpublished
Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular and cellular consequences of Zika virus circulating in Brazil to the human brain development have not been studied yet. Here we describe alterations in human neurospheres derived from neural stem cells infected with Brazilian ZIKV. Combined proteomics and mRNA transcriptional profile analyses showed that Brazilian ZIKV, prior to induce cell death, alters cell cycle and halts neurogenic
more » ... d halts neurogenic programmes, in addition to regulate transcription and protein translation due to viral replication. These results point to biological mechanisms potentially implicated in brain malformations as a result of ZIKV congenital infection.
doi:10.7287/peerj.preprints.2033 fatcat:elmrgqgr7rgf3fz54sg7uqdhfm