Use of synthetic oligonucleotide DNA probes for the identification of Bacteroides gingivalis

B J Moncla, P Braham, K Dix, S Watanabe, D Schwartz
1990 Journal of Clinical Microbiology  
Six different oligonucleotide probes complementary to the hypervariable regions of 16S rRNA of Bacteroides gingivalis were tested for specificity and sensitivity against 77 field strains of B. gingivalis and 105 strains of 12 other Bacteroides species. The data demonstrated that these probes were very specific (range, 0.85 to 1.00) and sensitive (1.00). Some limited cross-reactions with other Bacteroides species were observed. Four of these probes should be useful for rapid detection and
more » ... ication of B. gingivalis. 324 Vol. 28, No. 2 on May 5, 2020 by guest Downloaded from tion in human periodontal disease.
doi:10.1128/jcm.28.2.324-327.1990 fatcat:tapahk5jbrgutcrril6573r5nu