The present study was conducted to determine the localization of Brucella species within different aborted tissues. Another objective was to determine its cross species transmission of Brucella in three main districts of Punjab, Pakistan with significant bovine population. The results revealed the presence of Brucella abortus (23.3%) only and none of bovine fetus was positive for Brucella melitensis (0%) while lung was the most prevalent targeted tissue for Brucella as compare to liver and

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... ch respectively in both cattle and buffaloes fetuses. The results also indicated increased Brucella incidence in cattle fetuses (30 & 13.3%) than buffalo fetuses (16.6 & 6.66%) (P=0.034) by both AMOS PCR and IHC respectively. We also found significantly more positive cases by AMOS PCR than IHC (P=0.024). These novel findings about best specimen selection for isolation of Brucella and absence of Brucella melitensis in bovines of Punjab Pakistan can be helpful in designing brucellosis control programs.