Microbiological Evaluation of Minocycline Microspheres on the Type and Number of Bacteria in the Treatment of Chronic Periodontitis Using Multiplex PCR and Viable Counting: An in-Vivo study

Marwa Azab, Marina Naguib, Hany Shalaby
2022 Records of Pharmaceutical and Biomedical Sciences  
Periodontitis is an inflammatory infection of the periodontal tissue that, if left untreated, can lead to tooth loosening and, finally, tooth loss. The current study goals to identify the effectiveness of minocycline microspheres (Arestin) in the cure of chronic periodontitis, where forty chronic periodontitis patients (17 males and 23 females) between the ages of 20 and 45 were included. Patients were randomly assigned to one of two groups. 20 patients in Group I (the test group) received
more » ... in-based scaling and root planning (SRP) therapy (Minocycline HCl microspheres). 20 patients in Group II (Control group) who received only scaling and root planning (SRP) treatment. In order to plating on (non-selective) Anaerobic Thioglycolate Medium Base (ATMB) agar plates supplemented with horse serum (5 percent) and kanamycin-vancomycin-bacitracin, which is used for the selective recovery of obligatory Gram-negative rods (anaerobic) for colony forming unit calculations, microbiological samples were taken from both groups before treatment (baseline) and on days 30, 90, and 180 after treatment. A Multiplex PCR was used to amplify the bacterial DNA from Tannerella forsythia (T.f.), Aggregatibacter actinomycete mcomitans (A.a.), Treponema denticola (T.d.),and Porphyromonas gingivalis (P.g.). Groups I and II had significantly lowed microbiological analysis. For individuals with chronic periodontitis, scaling and root planning using Arestin (microbeads containing minocycline hydrochloride) may be a novel and efficient therapeutic approach.
doi:10.21608/rpbs.2022.140001.1140 fatcat:lywnthwwv5faxdfqrkiu6vzpde