Rapid Tilt-Series Methods: The Future of Cryo-ET

Georges Chreifi, Songye Chen, Grant Jensen
2020 Microscopy and Microanalysis  
Cryo-electron tomography (Cryo-ET) has become an indispensable imaging technique to reveal the structures of biological complexes and the architectures of cells in a nearly native frozen-hydrated state. In Cryo-ET, a tilt-series is collected by tilting the sample around a tilt axis, acquiring 2D images at each discrete tilt angle. This tilt-series is then used to computationally reconstruct a 3D tomogram. One of the major challenges in Cryo-ET is throughput; a typical tilt-series takes an
more » ... ries takes an average of 30-40 minutes to collect, where the time is mostly spent on tracking tasks that ensure the target remains centered in the field of view (FoV). We recently developed methods
doi:10.1017/s1431927620023491 fatcat:izus2ehwnbbpjnxu4446wwy74e