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___________________________________ Josep Comeron ___________________________________ John Manak ii To my family. iii ACKNOWLEDGMENTS I would like to thank my family first and foremost. Without their love and support, this thesis would not have been possible. Thank you to all of the members of the Bhattacharya lab:doi:10.17077/etd.4q66unr6 fatcat:dllnqwnn4zhejc74t4lglg4rva