The Negative Acts Questionnaire (NAQ-R) is a widely used measure of workplace bullying. However, studies examining its psychometric properties for use among Malaysian junior doctors have not been published. The study aims to determine its validity and reliability in assessing bullying among a Malaysian sample. Methods: The NAQ-R was administered to 1,119 junior doctors working in twelve government hospitals accredited for housemanship training within the central zone of Malaysia. A subset of

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... ticipants (n=50) completed the NAQ-R twice at an interval of two weeks. Exploratory factor analysis was conducted to assess construct validity, using polychoric factor analysis with varimax rotation. To determine reliability, Cronbach's alpha was computed to assess internal consistency reliability, while intraclass correlation coefficient was calculated to examine test retest reliability. Results: Analysis yielded a one-factor structure of the NAQ-R, consistent with the interpretation of factors provided by the original instrument. The factor was labelled "workplace bullying" and accounted for 68.0% of the variance in the junior doctor group. Factor loadings ranged from 0.68 to 0.90. The Cronbach's alpha for the NAQ-R items ranged from 0.96 to 0.97, and the Cronbach's alpha for the overall scale was 0.97. Meanwhile, the ICC for the NAQ-R items ranged from 45.6% to 93.7%, and the ICC for NAQ-R total score was 93.4%. These indices denote high internal consistency reliability and excellent test-retest reliability respectively. Conclusion: The NAQ-R has adequate psychometric properties and can be used to measure workplace bullying among Malaysian junior doctors.