Cardiomyocyte apoptosis and ventricular remodeling after myocardial infarction in rats

Eeva Palojoki, Antti Saraste, Anders Eriksson, Kari Pulkki, Markku Kallajoki, Liisa-Maria Voipio-Pulkki, Ilkka Tikkanen
2001 American Journal of Physiology. Heart and Circulatory Physiology  
and Ilkka Tikkanen. Cardiomyocyte apoptosis and ventricular remodeling after myocardial infarction in rats. Am J Physiol Heart Circ Physiol 280: H2726-H2731, 2001.-We investigated the role of cardiomyocyte apoptosis in the remodeling of the left ventricle from 24 h to 12 wk after myocardial infarction in the rat. Infarct size planimetry, quantification of cardiomyocyte apoptosis, terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) methodology, and echocardiography (left
more » ... ocardiography (left ventricular diastolic diameter and ejection fraction) were performed. Sham-operated animals showed low rates of cardiomyocyte apoptosis (0.03%) and no change in diastolic diameter or ejection fraction during the study. Twenty-four hours after infarction, TUNEL positivity was high in the infarct areas (1.4%) and border zones (4.9%). It declined to 0.34% (P Ͻ 0.01 vs. sham) at 4 wk and 0.10% at 12 wk in the border zones. In the remote myocardium, cardiomyocyte apoptosis increased to 0.07% (P ϭ 0.03 vs. sham) on day 1 and remained on the same level up to 4 wk. The increase in diastolic diameter 1-4 wk after infarction correlated (r ϭ 0.60, P Ͻ 0.01) with cardiomyocyte apoptosis in the noninfarcted myocardium, which quantitatively contributed most (Ͼ50%) to the apoptotic cell loss by 4 wk. ischemia
doi:10.1152/ajpheart.2001.280.6.h2726 pmid:11356629 fatcat:jbequfyf2ba7jf4br3mwnaxnfy