Mathematical Models to Predict Staphylococcus aureus Growth on Processed Cheeses

Kyungmi Kim, Heeyoung Lee, Jinsan Moon, Youngjo Kim, Eunjeong Heo, Hyunjung Park, Yohan Yoon
2013 Journal of Food Hygiene and Safety  
This study developed predictive models for the kinetic behavior of Staphylococcus aureus on pro- cessed cheeses. Mozzarella slice cheese and cheddar slice cheese were inoculated with 0.1 ml of a S. aureus strain mixture (ATCC13565, ATCC14458, ATCC23235, ATCC27664, and NCCP10826). The inoculated samples were then stored at 4 o C (1440 h), 15 o C (288 h), 25 o C (72 h), and 30 o C (48 h), and the growth of all bacteria and of S. aureus were enumerated on tryptic soy agar and mannitol salt agar,
more » ... spectively. The Baranyi model was fitted to the growth data of S. aureus to calculate growth rate (µ max ; log CFU·g −1 ·h −1 ), lag phase duration (LPD; h), lower asymptote (log CFU/ g), and upper asymptote (log CFU/g). The growth parameters were further analyzed using the square root model as a function of temperature. The model performance was validated with observed data, and the root mean square error (RMSE) was calculated. At 4 o C, S. aureus cell growth was not observed on either processed cheese, but S. aureus growth on the mozzarella and cheddar cheeses was observed at 15 o C, 25 o C, and 30 o C. The µ max values increased, but LPD values decreased as storage temperature increased. In addition, the developed models showed acceptable performance (RMSE = 0.3500-0.5344). This result indicates that the developed kinetic model should be useful in describing the growth pattern of S. aureus in processed cheeses.
doi:10.13103/jfhs.2013.28.3.217 fatcat:rrs7tflyxzc43k2cgad6tqrxpq