In Vitro Studies on the Influence of Different Concentrations of Growth Regulators on Economically Important Orchid, Vanilla planifolia

Vijaykumar Biradar, Ashashree Inamdar, Aswini Shamse, M.S. Patil
2016 International Journal of Current Microbiology and Applied Sciences  
Vanilla is propagated by stem-cuttings. Collection of stem-cuttings from the mother plants to arrest of growth, development and yield. In order to meet the demand for propagates, the rapid regeneration method of this species is essential. Micropropogation of Vanilla planifolia has been reported through callus culture, protocorn, root tips and axillary bud explants. Even though few reports are available on in vitro propagation, the protocols are complicated, hence in this report a simple,
more » ... rt a simple, economical, rapidly multiplying and highly reproducible protocol for large scale micropropogation is made. In this work, the protocol developed for shoot tip culture of Vanilla Planifolia on Murashige and Skoog"s medium supplemented with different growth regulators. In this experiment the shoot induction is studied on MS medium supplemented with different concentration of BAP, in which it is showing average leaf production on BA 0.5 mg/liter. we have also studied effect of different concentration of BAP & coconut water (CW) on average leaf formation in which BAP 1.0 mg/liter with 1.5% coconut water showing average leaf formation and also one of the MS basal medium which is supplemented with different growth regulators that is IAA, Kinetin, BAP, D-Biotin, Ca-Pentatrenate, is also showing the maximum leaf formation. K e y w o r d s Vanilla planifolia, Micropropagation, Growth regulators, BAP, Coconut water, Callus, MS medium.
doi:10.20546/ijcmas.2016.509.035 fatcat:l4ydtnnewrawlmvsdzkdbdyqhu