Studies on Certain Aspects of the Dynamics of Cholesterol Deposition in Rabbit Arterial Tissue

S. Mukherjee, C. Mukherjee
1979 VIIth International Congress on Thrombosis and Haemostasis   unpublished
Rabbits were maintained on a 1% cholesterol diet for 30, 60 and 90 days and jsotope labeling of aorta and serum was attained by feeding 100 mc of 4-14C-cholesterolduring the last 4 days before sacrifice of each group. Labeled aorta and low-density lipoprotéine (LDL) isolated from sera of the various diet regimens were employed for in vitro study of rates of flux of free and esterifiedcholesterol by incubating respectively with LDL and aorta of a rabbitmaintained for similar periods on above
more » ... eriods on above diet but given no radiocholesterol. Influx of free and ester cholesterol into thoracic aorta of hypercholesterolemia rabbit were respectively 6 and 11 times that obtained with tissues isolated from norraocholesterolemic animals. With progressive Ingestion of hypercholesterolemia diet, a gradual increase in rate of influx with concomitant lowering of efflux rate was noticed for both free and ester cholesterol. Studies on percentage clearance of labeled cholesterol from aorta of hypercholesterolemic rabbits into serum LDL indicate that 25 to 30 percent of radioactivity of free cholesterol is removed by LDL as against 2 to 4.5 percent of ester cholesterol radioactivity. Experiments carried out with partially de-lipidated LDL of sera hypercholesterolemic rabbits reveal that efflux of cholesterol from aorta is markedly increased.
doi:10.1055/s-0039-1687615 fatcat:fe2s2n34abbd5lciddjw5f4qji