1 1 O bj e cti ve : O bj e cti ve : To evaluate the expression of miR-155, nuclear factor kappa B (NF-κB) and soluble intercellular adhesion molecule-1 (sICAM-1) in the peripheral blood of patients with type 2 diabetes mellitus (T2DM) and assess its relationship with diabetic vascular lesions. Me thods: Me thods: A total of 165 T2DM patients and 60 healthy patients were included. Patients were divided into two groups: a simple T2DM group and a T2DM with vascular lesion group. The latter was

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... her divided into a microvascular lesion group, a macrovascular lesion group and a microvascular combined with macrovascular lesion group. Albuminuria levels were divided into three groups as follows: <30 mg / 24 h, normal albuminuria group (NAU group); 30-300 mg / 24 h, microalbuminuria group (MAU group); and ≥300 mg / 24 h, large albuminuria group (LAU group). Levels of peripheral blood miR-155, NF-κB, and sICAM-1 were detected by RT-PCR [SE . Re sul ts: Re sul ts: ①Compared with that in the control group, the expression of miR-155, NF-κB, and sICAM-1 in the T2DM group was significantly increased [(1.P<0.05]. ②The levels of miR-155, NF-κB, and sICAM-1 were significantly higher in the T2DM vascular lesion group than in the simple T2DM group [(1.P<0.05]. ③The expression of miR-155 in the microvascular combined with macrovascular lesion group was higher than that in the macrovascular lesion group [(2.36±0.61) vs. (1.77±0.59); P<0.05], and the level of NF-κB in the former group was significantly higher than those in the microvascular and macrovascular lesion groups (P<0.05). ④Compared with that in the NAU group, the level of miR-155 was significantly increased in the MAU and LAU groups [(1.04±0.20) vs. (1.41±0.49) vs. (2.64±0.52), respectively; p<0.05], and the miR-155 level in the LAU group was higher than that in the MAU group (P<0.05). ⑤Pearson correlation analysis showed that miR-155 was positively correlated with NF-κB and sICAM-1 (R=0.259, 0.67; P<0.01). Concl usi ons: Concl usi ons: miR-155 expression was increased in T2DM patients and vascular lesions, consistent with the increasing trends observed for NF-κB and sICAM-1. These findings suggest that miR-155 may be involved in the occurrence and development of diabetic chronic vascular disease. Subj e cts a nd Me thods Subj e cts a nd Me thods One hundred sixty-five T2DM patients were randomly selected at the endocrinology department