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A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based on spectral data, is less error-prone than intensity-based visualization and evaluation methods, and provides quantitative information on the composition of the sample. The methoddoi:10.1117/1.jbo.17.10.106011 pmid:23224010 fatcat:iu6h4qfb7fbpjczehmcefr63xy