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Background: The analytical process, especially reagent-related factors, demonstrates a significant effect on clinical chemistry analyzer. Therefore, it should have our attention both in total laboratory system (TLA) and modular automation (MA). The reagent-loading mode of modular analyzer influences testing turnaround time (tTAT) and the cost.Methods: Parameters were simulated by cobas 8000 workflow simulator for reagent-loading manner with at least three positions (Pattern 1), thedoi:10.21203/rs.3.rs-18675/v1 fatcat:pz22pn6qrfckjfdnpgym5ifrwa