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Protoplast Isolation from Shoots of Asparagus Cultures
International journal of plant sciences
Experiments to maximize the isolation and purification of viable protoplasts from shoot cultures of asparagus (Asparagus oficitzalis L.) were conducted. Important factors for high yield of viable protoplasts included: the use of in vitro etiolated shoots as source material; 0.6 M glucose as an osmoticum in a modified KM medium; a combination of pectinase, cellulase, and hemicellulase, each at 1% (wlv) for enzymatic digestion of cell walls; and physical factors such as the volume of enzymedoi:10.1086/297464 fatcat:3pfpukw5dbbflbwukrfdj7gmky