COMPARATIVE STUDY OF DYE-LOADED LIPOSOME ACCUMULATION IN SENSITIVE AND RESISTANT HUMAN BREAST CANCER CELLS

S Yefimova, I Kurilchenko, T Tkacheva, V Rozhkov, A Sorokin, N Lukianova, N Bezdenezhnykh, Yu Malyukin, V Chekhun
2012 Experimental Oncology   unpublished
The aim of this research is to study the dynamics and efficiency of liposome accumulation in sensitive and resistant human breast cancer cells. Methods: Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used. Results: The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal
more » ... gy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness. Conclusion: Obtained results show that resistant cells accumulate dye-loaded liposomes more effectively and reveal more effective dye molecule cellular uptake.
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