Imaging pancreatic β-cells in the intact pancreas

Manami Hara, Restituto F. Dizon, Benjamin S. Glick, Catherine S. Lee, Klaus H. Kaestner, David W. Piston, Vytautas P. Bindokas
2006 American Journal of Physiology. Endocrinology and Metabolism  
We have developed a method to visualize fluorescent protein-labeled ␤-cells in the intact pancreas through combined reflection and confocal imaging. This method provides a 3-D view of the ␤-cells in situ. Imaging of the pancreas from mouse insulin I promoter (MIP)-green (GFP) and red fluorescent protein (RFP) transgenic mice shows that islets, ␤-cell clusters, and single ␤-cells are not evenly distributed but are aligned along the large blood vessels. We also observe the solitary ␤-cells in
more » ... tary ␤-cells in both fetal and adult mice and along the pancreatic and common bile ducts. We have imaged the developing endocrine cells in the embryos using neurogenin-3 (Ngn3)-GFP mice crossed with MIP-RFP mice. The dual-color-coded pancreas from embryos (E15.5) shows a large number of green Ngn3-expressing proendocrine cells with a smaller number of red ␤-cells. The imaging technique that we have developed, coupled with the transgenic mice in which ␤-cells and ␤-cell progenitors are labeled with different fluorescent proteins, will be useful for studying pancreatic development and function in normal and disease states.
doi:10.1152/ajpendo.00365.2005 pmid:16368785 fatcat:vbrbzhp5jfabpmkkexk62ncddu