Original Article Loss of SOX5 protein expression by RNAi in osteosarcoma cells suppresses cell proliferation and invasion

Shuwei Zhang, Yichi Zhou, Yuanyu Zha, Yang Yang, Linlong Wang, Jingfeng Li, Wei Jin
2017 Int J Clin Exp Pathol   unpublished
Sex determining region Y-box protein 5 (SOX5) is involved in the regulation of embryonic development and associated with various types of cancers. However, the expression and biological function of SOX5 in osteosarcoma remains to be investigated. Here, we found that SOX5 mRNA was significantly elevated in osteosarcoma tissues compared with bone cyst tissues. In vitro experiments demonstrated that knockdown of SOX5 by RNA interference in two osteosarcoma cell lines, MG63 and U2OS cells,
more » ... cell proliferation, G1/S cell cycle transition, migration and invasion. In addition, knockdown of SOX5 in osteosarcoma cells reduced the expression of proteins associated with G1/S cell cycle transition (CDK2 and CDC25A) and invasion (MMP-2, MMP-9, Twist1 and Snail1). Our results indicate for the first time that SOX5 is a potential diagnostic biomarker and a therapeutic target for osteosarcoma.