Mms6 expression in adipose-derived mesenchymal stem cells: development of biogenic magnetic nanoparticles and Its therapeutic potential [article]

Fransiscus Fiano Anthony Kerans, University Of Edinburgh, University Of Edinburgh, Donald Salter, Chris Boyd
2020
Magnetic nanoparticles (MNP) are attracting interest for a range of biomedical applications being used either alone or as part of cell-based therapies. An area of particular interest is magnetic nanoparticle-mediated hyperthermia (MNHT), when MNP absorb energy from alternating magnetic fields (AMF) and transform this energy into heat which results in cancer cell death. While promising, the use of MNP for diagnosis and therapy has been limited by their rapid removal from the blood and biological
more » ... lood and biological barriers at the tissue and cellular levels. Moreover, MNP may have adverse side effects when used clinically. To overcome these problems there is increasing interest in the development of cellbased strategies to deliver MNP. Current strategies include combining commercially available MNP with mesenchymal stem cells (MSC), as these cells can migrate to sites of tissue injury and tumor growth. However, problems with MNP cytotoxicity have hindered progress in this area and need to be overcome. Therefore, the ultimate aim of this PhD project was to find an alternative way to develop MSC that contain MNP using a genetic engineering approach by which the cells can be induced to stably produce biogenic MNP and to establish whether such an approach could be of value for MNHT for cancer treatment. To achieve this goal, the experiments conducted in this PhD project involved transfection of adipose-derived mesenchymal stem cells (AD-MSC) to introduce a synthetic magnetic gene, mms6, into the cells. The gene is originally derived from Magnetospirillum magneticum AMB-1, a genus of magnetotactic bacteria (MTB), which has unique intracellular structures called magnetosomes. The magnetosomes contain iron-rich magnetic nanoparticles that are enclosed within a lipid bilayer membrane. During the formation of magnetosome, mms6 has been known for its key role in the formation of uniform isomorphic magnetite nano-crystals and helps regulate the crystal morphology of magnetite. Due to the unique feature of this gene, therefore the novelty of thi [...]
doi:10.7488/era/303 fatcat:c3dekg62yrduvo6ihmnsxapeza