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Sequence-specific analysis of oligodeoxynucleotides by precipitate-amplified surface plasmon resonance measurements
Frontiers in Bioscience
A flow injection SPR spectrometer is used to detect DNA hybridization via amplification by an enzymecatalyzed precipitation reaction. Hybridization of an oligodeoxynucleotide (ODN) target with a surfaceconfined ODN probe, followed by hybridization between the overhang on the resultant duplex and a biotin-tagged ODN, yields a "sandwich" complex (a three-component double-stranded). A streptavidin-horseradish peroxidase conjugate (SA-HRP) can be attached to the sandwich complex-covered surface viadoi:10.2741/2552 pmid:17569634 fatcat:tyh65sysfbcjza3h4q24vzfeq4