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Enzyme immunoassay measurements of the molting hormone in different post-embryonic stages of two mosquito species, Culex pipiens and Culiseta longiareolata

Tine-Djebbar
2011 African Journal of Biotechnology  

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Due to their large geographical distribution, abundance and harmfulness, Culex pipiens L. and Culiseta longiareolata Macquart are the two most remarkable mosquito species in Algeria. The nature and the level of ecdysteroids from whole body extracts of different post-embryonic stages of the two mosquito species was made by an enzyme-immunoassay (EIA) using two specific antibodies, the rat monoclonal EC 19 antibody showing a high affinity for 20-hydroxyecdysone (20E) and the rabbit polyclonal B
more » ... tibody with a strong affinity for ecdysone (E). EIA measurements confirmed the presence of two main hormones in the two mosquito species: E and 20E. Moreover, there is a predominance of 20E.
doi:10.5897/ajb11.2085 fatcat:3oml5s4j35cmvno3ww3h7fgp4a
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Tine-Djebbar. "Enzyme immunoassay measurements of the molting hormone in different post-embryonic stages of two mosquito species, Culex pipiens and Culiseta longiareolata." African Journal of Biotechnology 10.67 (2011)