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Effect of endoplasmic reticulum stress on uremic serum-induced endothelial cell dysfunction
Medical Journal of Chinese People's Liberation Army
Objective To investigate the effect of endoplasmic reticulum stress in uremic serum-induced endothelial cell dysfunction.Methods Human umbilical vein endothelial cells(hUVECs) were cultured in growth medium with the addition of uremic serum collected from uremic patients in different concentrations,or normal serum collected from normal adults for 24h.The cell viability was then tested by MTT assay and the cell cycle was analyzed by flow cytometry(FCM).Hoechst33342 assay was used to analyze thedoaj:1ba83d7072054564ab7872ba37c35ea3 fatcat:d7eddfm2wvho7bul4a6o6mvkhm