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The Plant Cell
Arabidopsis GF14w was originally described because of its apparent association with a DNA-protein complex; it is a member of the 14-3-3 kinase regulatory protein family that is conserved throughout eukaryotes. Here, we demonstrated that recombinant GF14o isexpressed in Escherichia colias a dimer. Blot binding and electrophoretic mobility shift analyses indicated that GFl4o binds calcium. Equilibrium dialysis further demonstrated that G F l h binds an equimolar amount of calcium with an apparentdoi:10.2307/3869930 pmid:8205002 fatcat:5ftp6zt2offhlgl6onwlkh3f5e